GENIE® Electrophoretic Transfer

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Genie Blotter Instructions (pdf version)

Ordering Information

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The GENIE is the proven electrophoretic transfer apparatus incorporating state-of-the-art plate electrodes for the ultimate in field uniformity. Its design allows for a very close positioning of the electrodes which, when coupled with a low voltage power source such as a battery charger, creates a high field in volts/cm. The result is exceptionally rapid and exact electrophoretic transfer. With the GENIE, it is possible to obtain superb Western blots in as little as 30 minutes at 24 volts¹,² and Northern blots in 1.5 hours from agarose³ or acrylamide⁴ gels. The rapid Southern procedure does not require gel pretreatment and is useful for labs that require extreme sensitivity to detect limited copy number sequences⁵ or that perform numerous Southern blots for the authentication of library clones.^6,7 Even megabase DNA from CHEF gels can be blotted in one hour.^8,9 Electrophoretic blots have a much lower background than vacuum, pressure or capillary blots.^3,5 The uniform field facilitates quantitative Southern⁵, Western⁵, and Northern⁴ blots.

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Western Blot	Southern Blot

Click for larger view	Agarose CHEF gel blotted one hour at 12 volts using an Idea Scientific ROYAL GENIE. Blot was soaked in a 0.4 N NaOH ten minutes and probed. Data courtesy of Dr. Robert Haire, All Childrens Hospital, St Petersburg, Florida

LOAD HORIZONTALLY...BLOT VERTICALLY

Conventional tank-type blotters are not only notoriously slow "buffer hogs," but their edge-supported blotting cassettes allow gel slumping during transfer. Our research scientists have also noted that semi-dry blotters, on the other hand, trap electrode gases in pockets that disrupt the field, require frequent replacement of electrodes with poor durability, and often "burn" blots. The GENIE, designed with an insertable tray for buffer-submerged bubble-free loading, is the only blotter to eliminate these problems. Simply slide the loaded tray into the tray holder while the apparatus is in a horizontal position, return it to its upright vertical position, and begin blotting. The plate electrodes support the gel and provide a uniform field.

References

1. Rapid High Resolution Western Blotting: From Gel to Image in a Single Day, Klapper, A., et al., Biotechniques, 12, (1992), pp. 651-654.

The preceding reference was originally published in BioTechniques 12:5,650-653. Appearing with permission from Eaton Publishing. It is available is an Acrobat PDF file. Use the Free Adobe Acrobat Reader to view it. Please download the latest version for your particular computing platform.

2. Rickard, J.E., and Kreis, T.E., J. Cell Biology, 110, (1990), pp. 1623-1633.

3. An Improved Rapid Northern Protocol, Kevil, C.G., et. al., B.B.R.C., 238, (1997), pp. 277-279.

4. Hajnsdorf, E. et. al. The EMBO Journal. 14, (1994), pp. 3368-3377.

5. Li, S., et.al. J. Exp. Med., 189, (1999) pp. 1399-1412.

6. Amemiya, C.T. and Zon, L.I., Genomics 58, (1999) pp 211-213.

7. Amemiya, C.T. et. al., Gene, 272, (2001) pp.283-289.

8. Haire, R.N., personal communication.

9. Paul, A. et al. The Plant Cell, 10, (1998) pp 1349-1359.

Other references can be found in the Bibliography.

ORDERING INFORMATION

The GENIE is offered in five sizes. The standard alloy electrodes are adequate for most routine blotting. For difficult blots (high molecular weight proteins, large nucleic acids, etc.), order an apparatus with a platinized titanium anode. Platinized titanium anodes delivered after October 1, 1997 have a manufacturing date engraved on them, a thicker coating than earlier coated anodes, and have a five year warranty. Blotters include apparatus, two electrodes, pads, screens and instructions. Cables are not included with apparatus, but are permanently attached to the power supplies. Order power supplies below.

GENIE POWER SUPPLIES

These modified battery chargers are the perfect power supplies for GENIE blotters. They are designed to produce very specific voltages, giving much greater reproducibility than laboratory power supplies. 110 volt input power supplies (for North American use).

INTERNATIONAL 12 VOLT GENIE POWER SUPPLY (for export)

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