Take a PubMed online look at some data produced by Idea Scientific equipment.
Put the PMID number in the search window at pubmed.gov
"Aging, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function" Russ, D.W., et.al., Acta Physiol, Vol. 201, pp 391-403 (2011). Figure 3 shows a nice Genie blot of 565 kD ryanodyne receptor. The author reports adding 0.01% SDS to the blotting buffer, and blotting overnight at 15 volts. (PMID: 20874807)
"Myotendinous Junction Defects and Reduced Force Transmission in Mice that Lack alpha Integrin and Utrophin" Welser, J.V., et. al. Am. J. Pathol., Vol. 175, pp1545-1554 (2009). Figure 4 shows sharp-banded Genie western blot of 427 kD Dystrophin. (PMID: 19729483)
"Occluden oligomeric assembly at tight junctions of the blood-brain barrier is disrupted by peripheral inflammatory hyperalgesia" McCaffrey, G. et. al., J. Neurochem., Vol. 106, pp. 2395-2409 (2008). Figure 5 shows the sharpness of Genie blots can resolve 220 kD zonula occludens-1 isoforms. (PMID: 18647175)
"Multiple Mechanisms Contribute to Inhibit Transcription in Response to DNA Damage" Heine, G.F. , et. al., J. Biol. Chem., Vol. 283, pp 9555-9561 (2008). Figures 1, 4, 5 and 6 show nice resolution of 210 kD Rpb1 and phosphorylated Rpb1. (PMID: 18281289)
"Age-Related Dystrophin-Glycoprotein Complex Structure and Function in the Rat Extensor Digitorum Longus and Soleus Muscle" Rice, K.M. et. al., J. of Gerontology, Vol.61A, pp.1119-1129 (2006). Figure 5 shows quantitative detection of dystroglycans (250kD) on Genie western blots. (PMID: 17167152)
"Tyrosine kinase inhibitors impair fibroblast growth factor signaling in coronary endothelial cells" Hawker, J.R. Jr. and Granger H.J Am.J. Physiol. Vol 266, pp H107-H120 (1994). Figures 5 and 6 show Genie blots are sharp enough to resolve doublets 2 kD apart on blots of proteins up to 240 kD. (PMID: 8304491)
"Autoantibody research using the Surf-Blot System" (2013) Review. www.biocompare.com/Product-Reviews/140203-Autoantibody-research-using-the-Surf-Blot-System/
"Immunogenicity of mycobacterial vesicles in humans: identification of a new tuberculosis antibody biomarker" Ziegenbalg, A. et.al. Tuberculosis, Vol. 93, 448-455 (2013) Surf-Blot data in figure 4 (PMID 23562367)
"Optimal Long-Term Humoral Responses to Replication-Defective Herpes Simplex Virus Require CD21/CD35 Complement Receptor Expression on Stromal Cells", Brockman, Mark A., et. al., J. Virol, Vol. 80, pp. 7111-7117 (2006). Figure 3 of this paper shows testing of mouse sera screening on a western blot using a Surf-Blot. (PMID: 16809316)
"Human Stx-2-Specific Monoclonal Antibodies Prevent Systemic Complications of Escherichia coli O157:H7 Infection", Mukherjee, J., et. al., Infection and Immunity, Vol 70, pp. 612-619 (2002). Fig 1 of this paper shows monoclonal antibody screening on an intact western blot. (PMID:11796590)
"Role of protein kinase C in light adaptation of molluscan microvillar photoreceptors", Piccoli, G. et al., Journal of Physiology, Vol. 543.2, pp. 481-494 (2002). Figure 3 of this paper shows polyclonal antibody screening with a Surf-Blot. (PMID: 12205183)
"Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against different protein subunits". Sinnarajah, S. et. al., FEBS Letters, Vol. 426, pp. 377-380 (1998). Surf-Blot used for Figure 3A. (PMID: 9600270)
"Merlin, the Neurofibromatosis Type 2 Gene Product, and beta1 Integrin Associate in Isolated and Differentiating Schwann Cells", Obremski, V.J., et. al., Journal of Neurobiology, Vol. 37, pp. 487-501 (1998). Surf-Blot used for figures 1,2,3,5, and 6. (PMID: 9858254)
"The Grid-Blot: A Procedure for Screening Large Numbers of Monoclonal Antibodies for Specificity to Native and Denatured Proteins", Lane, R.D., et. al., Hybridoma, Vol. 8, pp. 661-669 (1989). This paper describes the procedure for Grid-Blot screening, which does not require electrophoresis or blotting. (PMID: 2693340)
"Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross reactivity", Reese, G. et. al., Journal of Chromatography B, Vol. 756, pp. 151-156 (2001). This refinement of the Grid-Blot procedure examines meat allegies in allergic patients. The procedure uses approximately ten times less antibody than ELISA sera screening. (PMID: 11419706)
"Identification of Continuous, Allergenic Regions of the Major Shrimp Allergen Pen a 1 Tropomyosin" Ayuso, R., et. al., International Journal of Allergy and Immunology, Vol. 127, pp. 27-37 (2002). Forty-six overlapping peptides spanning the entire tropomyosin molecule are tested with serum from shrimp-allergic patients to map the allergenic regions of tropomyosin. (PMID: 11893851)
"Rapid High Resolution Western Blotting: From Gel to Image in a Single Day", Klapper, A. et. al., Biotechniques, Vol. 12, pp.650-654 (1992). The 30 minute GENIE transfer step helps shorten the entire Western procedure. (PMID: 1515130)
"Identification of a novel nucleotide-sensitive microtubule-binding protein in HeLa cells", Rickard, J.E. and Kreis, T.E., Journal of Cell Biology, Vol. 110, pp 1623-1633 (1990). Figures 1, 2 and 3 show that a 170kD protein can be blotted in 30 minutes with a GENIE. (PMID: 1970824)
"The P190, P210, and P230 Forms of the BCR/ABL Oncogene Induce a Similar Chronic Myeloid Leukemia-like Syndrome in Mice but Have Different Lymphoid Leukemogenic Activity", Li, S., et. al., J. Exp. Med., Vol. 189, pp. 1399-1412 (1999). This paper from laboratories at the Harvard Center for Blood Research, Dana-Farber Cancer Institute and the Whitehead Institute shows the high sensitivity and low background of electroblotting with the Genie. Dr. Van Etten reported that he can detect one-twentieth of a gene copy per diploid genome. (PMID:10224280)
"Extension of Life-Span by Introduction of Telomerase into Normal Human Cells", Bodnar, A. G. et. al., Science, Vol. 279, pp. 349-352 (1998). This paper was the most highly referenced Southern blot paper of 1998. (PMID: 9454332)
"Higher Order Chromatin Structures in Maize and Arabidopsis", Paul, A. and Ferl, R.J., The Plant Cell, Vol.10, pp. 1349-1359 (1998). This paper demonstrates the ability to electroblot large, intact, DNA from a CHEF gel without having to process the gel before blotting. (PMID: 9707534)
"Generation of a Zebrafish P1 Artificial Chromosome Library", Amemiya, C.T. and Zon, L.I., Genomics, Vol. 58, pp. 211-213 (1999) and "Generation of a P1 artificial chromosome library of the Southern pufferfish", Amemiya, C.T., et. al., Gene, Vol. 272, pp. 283-289, (2001). These papers are from laboratories where authentication of library clones requires an extensive number of Southern blots. (PMID: 10366454 and 11470535)
"An Improved Rapid Northern Protocol", Kevil, K.G. et. al., Bioc. and Biop. Res. Comm., Vol. 238, pp. 277-279 (1997). GENIE transfer of RNA takes 1.5 hours. This procedure does not use formaldehyde or glyoxal. Background is so low that the blocking step is eliminated. (PMID: 9299493)
"Response of rat muscle to acute resistance exercise defined by transcriptional and translational profiling". Chen, Y. et. al., Journal of Physiology, Vol. 545, pp. 27-41 (2002). Figure 1 shows the distribution of mRNA sizes in polysomes using formaldehyde-agarose gels tansferred to nylon with the GENIE (publication says nitrocellulose membrane, that is incorrect). (PMID: 12433947)
"Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1". Barr, K. et. al. The FASEB Journal, Vol. 16, pp. 1879-1886 (2002). Westerns to nitrocellulose and formaldehyde agarose Northerns to nylon. (PMID: 12468452)
"Roles of RNase E, Rnase II and PNPase in the degradation of the rps transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant", Hajnsdorf, E., et. al., The EMBO Journal, Vol. 13, pp. 3368-3377 (1994). (PMID: 7519147)
"Comparative Structural Analysis of Nuclear RNse P RNAs from Yeast" Tranguch, A.J. and Engelke, D.R., Journal of Biological Chemistry, Vol. 268, pp. 14045-14053 (1993). (PMID: 8314772) 6% polyacrylamide RNA gels electrotransferred with the GENIE.
I was amazed at the results. Perfect transfer, no background and a great signal. It is incredibly easy to set up and will save my lab hundreds of man hours a year. Congrats on an excellent product.
I have tried quite a number of electrophoretic transfer units over the past 15 years, including tank and semi-dry styles. Although all work to one degree or another, my hands-down favorite unit is the Genie blotter from Idea Scientific