Ordering Information
Mini-Slab, the proven vertical mini-gel system, is operationally simple yet so comprehensive in application that it has been used for virtually every type of gel. With the capability of running 8-, 10- or 15-cm long gels, the versatile Mini-Slab is in operation in thousands of laboratories worldwide. The Mini-Slab, used principally for SDS-PAGE gels,1 has also been used for non-denaturing2 and acid-urea protein gels,3 nucleic acid and nucleo-protein agarose gels,4restriction digests,5 monoclonal antibody screening gels, 2-D gels6 and PCR gels.
The Mini-Slab is designed to eliminate the need for sealing gaskets, irreplaceable molded parts, and/or expensive and fragile notched plates. Auxiliary pumps are not needed as thin glass plates allow gels to air cool thoroughly so that buffer recirculation is unnecessary. Gel volume is low, which cuts the cost of chemicals, stains, de-stains and buffers. In addition, these thin gels can be air dried in an inexpensive Tut's Tomb Gel Drying Frames.
The Mini-Slab's running time is 20 to 90 minutes for most procedures. Gels are a scant 0.5 to 1.0 mm in thickness so that staining (or blotting) time is shorter than with larger gels. The optional cooling fan decreases the running time even further. The sensitivity of the small gel format allows for the detection of nanogram quantities of many proteins, so it is not necessary to first obtain high concentration samples.
All sets contain two combs and six 20-cm long, 4.0 mm wide spacers that can be cut to length for any size gel. Combs with ten wells have 6x16 mm wells, 14 and 18 well combs have 3.5x10 mm wells. All combs and spacers are hand matched to the nearest 0.01 mm in thickness. Rigid combs are treated with a permanent non-stick coating. WE ARE THE ONLY SUPPLIER OF THESE NON-STICK COMBS IN THE WORLD!
1. Matsudaira, P.T. and Burgess D.R., Anal. Biochem. 87,386-396 (1978). : Howe, C.L. and Mooseker, M.S., Journal of Cell Biology, 97, 974-985 (1983). : Hendricks, M. and Weintraub, H., Proc. Nat. Acad. Sci. USA, 78, 5633-5637 (1981). : Williman, T. and Szent-Gyorgyi, A.G., Biochemistry, 20, 1176-1187 (1981).
2. Francis, R.T. Jr. et.al., Journal of Chromatography, 298, 115-121 (1984).
3. Spiker, S., Anal. Biochem., 108, 263-265 (1980). : Richards, R.G. and Shaw, B.R., Anal. BIochem., 121, 69-82 (1982).
4. Yager, T.D. and Van Holde, K.E., Journal of Biological Chemistry, 259, 4212-4222 (1984).
5. Bailey, G.S. et. al., Biochemistry, 19, 5836-5842 (1980).
6. Davie, J.R., Anal. Biochem., 120, 276-281 (1982). : Porter, S. and Froehner, S.C., Journal of Biological Chemistry, 258, 10034-10040 (1983).
| Mini-Slab Vertical Electrophoresis | ||
|---|---|---|
| Catalog Number | Product Description | Price (U.S. $) |
| 1001X | COMPLETE MINI-SLAB SYSTEM. Includes MINI-SLAB, six combs (one each of the first six combs listed), 0.5 and 0.8 mm thick spacer material, eight 10x10 cm glass plates and instructions. | 295.00 |
| 2008 | COOLING FAN FOR THE MINI-SLAB. Allows a 3x power increase. 110 volt input. | 110.00 |
| 1018 | UNIVERSAL GRADIENT GEL POURING STAND. Allows one to pour up to 10 gradient or single concentration gels that are 8-, 10-, or 15-cm long. Includes 0.5 and 0.8mm thick spacer material and instructions. Order glass and combs separately. | 140.00 |
| 1003 | MULTIPLE GEL POUR STAND. Allows one to pour the separating gel of up to 15 single concentration gels simultaneously. For 8- or 10-cm long gels. | 30.00 |
| 1004 | GEL DRYING FRAME. Air dries gels without heat or vacuum. OTHER SIZES LISTED ELSEWHERE. | 28.00 |
| 1006 | PAIR OF CONNECTING CABLES. High quality cables with ends permanently molded to the wire. One is red, one black. Each cable has a banana plug on one end with a banana jack on the other. 140-cm long. | 50.00 |
| 2001 | STILTS PACKAGE. Everything needed to run 15 cm long gels on the MINI-SLAB. Includes six 10x15 cm glass plates, one STILTS adapter, and spacer material for 0.5 and 0.8 mm thick gels. #2008 fan can be mounted in STILTS. | 80.00 |
| 2002 | STILTS GLASS PLATES. Package of 12 10x15 cm quality ground edge glass plates. | 75.00 |
| 1017 | 10x10 cm GLASS PLATES. Package of 12 quality ground edge glass plates. | 70.00 |
| CAP-6 | CAPILLARY ADAPTER. MINI-SLAB upper reservoir for running six capillary gels. Usually used for the first dimension of 2-D gels. | 45.00 |
| V-6 | SEXTUPLETS. A six gel apparatus which can be used for the second dimension of 2-D gels. Includes: apparatus, twelve 10x10 cm glass plates, 1.0 and 1.5 mm thick spacers and instructions. | 400.00 |
| 7210 | GEL LOADING PIPET TIPS. Load gels as thin as 0.65 mm. Five racks of 200 per rack. | 70.00 |
| Set of two combs and six spacers | ||
|---|---|---|
| Catalog Number | Product Description | Price (U.S. $) |
| 1005 | 0.5 mm thick, 18 wells, Teflon. | 20.00 |
| 1041 | 0.5 mm thick, 18 wells, Rigid, non-stick. | 20.00 |
| 1035 | 0.5 mm thick, 10 wells, Rigid, non-stick. | 20.00 |
| 1042 | 0.8 mm thick, 18 wells, Teflon. | 20.00 |
| 1008 | 0.8 mm thick, 18 wells, Rigid, non-stick. | 20.00 |
| 1036 | 0.8 mm thick, 10 wells, Rigid, non-stick. | 20.00 |
| 1009 | 0.8 mm thick, 14 wells, Rigid, non-stick. | 20.00 |
| 1010 | 1.0 mm thick, 18 wells, Rigid, non-stick. | 20.00 |
| 1011 | 1.0 mm thick, 14 wells, Rigid, non-stick. | 20.00 |
| 1037 | 1.0 mm thick, 10 wells, Rigid, non-stick. | 20.00 |