Mini-Slab Vertical Electrophoresis

The First Mini-Gel

Instructions for Electrophoresis using the Mini-Slab (html version)

Instructions for Electrophoresis using the Mini-Slab (pdf version)


Gradient Gel Pouring Stand Instructions (html version)

Gradient Gel Pouring Stand Instructions (pdf version)


Multiple Gel Pouring Stand Instructions (html version)

Multiple Gel Pouring Stand Instructions (pdf version)


Ordering Information

Mini-Slab, the proven vertical mini-gel system, is operationally simple yet so comprehensive in application that it has been used for virtually every type of gel. With the capability of running 8-, 10- or 15-cm long gels, the versatile Mini-Slab is in operation in thousands of laboratories worldwide. The Mini-Slab, used principally for SDS-PAGE gels,1 has also been used for non-denaturing2 and acid-urea protein gels,3 nucleic acid and nucleo-protein agarose gels,4restriction digests,5 monoclonal antibody screening gels, 2-D gels6 and PCR gels.

INEXPENSIVE OPERATION

The Mini-Slab is designed to eliminate the need for sealing gaskets, irreplaceable molded parts, and/or expensive and fragile notched plates. Auxiliary pumps are not needed as thin glass plates allow gels to air cool thoroughly so that buffer recirculation is unnecessary. Gel volume is low, which cuts the cost of chemicals, stains, de-stains and buffers. In addition, these thin gels can be air dried in an inexpensive Tut's Tomb Gel Drying Frames.

RAPID ELECTROPHORESIS

The Mini-Slab's running time is 20 to 90 minutes for most procedures. Gels are a scant 0.5 to 1.0 mm in thickness so that staining (or blotting) time is shorter than with larger gels. The optional cooling fan decreases the running time even further. The sensitivity of the small gel format allows for the detection of nanogram quantities of many proteins, so it is not necessary to first obtain high concentration samples.

COMB AND SPACER SETS

All sets contain two combs and six 20-cm long, 4.0 mm wide spacers that can be cut to length for any size gel. Combs with ten wells have 6x16 mm wells, 14 and 18 well combs have 3.5x10 mm wells. All combs and spacers are hand matched to the nearest 0.01 mm in thickness. Rigid combs are treated with a permanent non-stick coating. WE ARE THE ONLY SUPPLIER OF THESE NON-STICK COMBS IN THE WORLD!


References

1. Matsudaira, P.T. and Burgess D.R., Anal. Biochem. 87,386-396 (1978). : Howe, C.L. and Mooseker, M.S., Journal of Cell Biology, 97, 974-985 (1983). : Hendricks, M. and Weintraub, H., Proc. Nat. Acad. Sci. USA, 78, 5633-5637 (1981). : Williman, T. and Szent-Gyorgyi, A.G., Biochemistry, 20, 1176-1187 (1981).

2. Francis, R.T. Jr. et.al., Journal of Chromatography, 298, 115-121 (1984).

3. Spiker, S., Anal. Biochem., 108, 263-265 (1980). : Richards, R.G. and Shaw, B.R., Anal. BIochem., 121, 69-82 (1982).

4. Yager, T.D. and Van Holde, K.E., Journal of Biological Chemistry, 259, 4212-4222 (1984).

5. Bailey, G.S. et. al., Biochemistry, 19, 5836-5842 (1980).

6. Davie, J.R., Anal. Biochem., 120, 276-281 (1982). : Porter, S. and Froehner, S.C., Journal of Biological Chemistry, 258, 10034-10040 (1983).

ORDERING INFORMATION

Mini-Slab Vertical Electrophoresis
Catalog Number Product Description Price (U.S. $)
1001X COMPLETE MINI-SLAB SYSTEM. Includes MINI-SLAB, six combs (one each of the first six combs listed), 0.5 and 0.8 mm thick spacer material, eight 10x10 cm glass plates and instructions. 295.00
2008 COOLING FAN FOR THE MINI-SLAB. Allows a 3x power increase. 110 volt input. 110.00
1018 UNIVERSAL GRADIENT GEL POURING STAND. Allows one to pour up to 10 gradient or single concentration gels that are 8-, 10-, or 15-cm long. Includes 0.5 and 0.8mm thick spacer material and instructions. Order glass and combs separately. 140.00
1003 MULTIPLE GEL POUR STAND. Allows one to pour the separating gel of up to 15 single concentration gels simultaneously. For 8- or 10-cm long gels. 30.00
1004 GEL DRYING FRAME. Air dries gels without heat or vacuum. OTHER SIZES LISTED ELSEWHERE. 28.00
1006 PAIR OF CONNECTING CABLES. High quality cables with ends permanently molded to the wire. One is red, one black. Each cable has a banana plug on one end with a banana jack on the other. 140-cm long. 25.00
2001 STILTS PACKAGE. Everything needed to run 15 cm long gels on the MINI-SLAB. Includes six 10x15 cm glass plates, one STILTS adapter, and spacer material for 0.5 and 0.8 mm thick gels. #2008 fan can be mounted in STILTS. 80.00
2002 STILTS GLASS PLATES. Package of 12 10x15 cm quality ground edge glass plates. 75.00
1017 10x10 cm GLASS PLATES. Package of 12 quality ground edge glass plates. 70.00
CAP-6 CAPILLARY ADAPTER. MINI-SLAB upper reservoir for running six capillary gels. Usually used for the first dimension of 2-D gels. 45.00
V-6 SEXTUPLETS. A six gel apparatus which can be used for the second dimension of 2-D gels. Includes: apparatus, twelve 10x10 cm glass plates, 1.0 and 1.5 mm thick spacers and instructions. 400.00
7210 GEL LOADING PIPET TIPS. Load gels as thin as 0.65 mm. Five racks of 200 per rack. 70.00

Comb and Spacer Sets
Catalog Number Product Description Price (U.S. $)
1005 0.5 mm thick, 18 wells, Teflon. 20.00
1041 0.5 mm thick, 18 wells, Rigid, non-stick. 20.00
1035 0.5 mm thick, 10 wells, Rigid, non-stick. 20.00
1042 0.8 mm thick, 18 wells, Teflon. 20.00
1008 0.8 mm thick, 18 wells, Rigid, non-stick. 20.00
1036 0.8 mm thick, 10 wells, Rigid, non-stick. 20.00
1009 0.8 mm thick, 14 wells, Rigid, non-stick. 20.00
1010 1.0 mm thick, 18 wells, Rigid, non-stick. 20.00
1011 1.0 mm thick, 14 wells, Rigid, non-stick. 20.00
1037 1.0 mm thick, 10 wells, Rigid, non-stick. 20.00

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